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より購入した ． dapi ii counterstainは abbott laboratories 東京  (Thermo Fisher)


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    Thermo Fisher より購入した ． dapi ii counterstainは abbott laboratories 東京
    より購入した ． Dapi Ii Counterstainは Abbott Laboratories 東京, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 23402 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/より購入した ． dapi ii counterstainは abbott laboratories 東京/product/Thermo Fisher
    Average 99 stars, based on 23402 article reviews
    より購入した ． dapi ii counterstainは abbott laboratories 東京 - by Bioz Stars, 2026-03
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    Morphological characterization of Ankrd31 –/– testes. (A) Comparative size analysis between WT and Ankrd31 –/– testes. Testis weight (mg) analysis was carried out on WT and Ankrd31 –/– testes ( n = 20). Data are reported as mean value ± S.E.M. (B,C) Defective spermatogenesis was observed by H&E staining of WT and Ankrd31 –/– Bouin’s fixed seminiferous tubule sections (7 μm) collected from 8-week-old testes. SPG, spermatogonia; SPC, spermatocytes; rSPT, round spermatids; eSPT, elongated spermatids (black-arrowheads). (D,E) Cells in metaphase are indicated by white arrowheads. (F) Quantification of seminiferous tubules containing spermatocytes in metaphase in WT and Ankrd31 –/– testes ( n = 10 for each genotype). The number of total tubules counted in five different animals for each genotype corresponded to n = 650. Data are expressed as percentage (%) of tubules containing cells in metaphase and reported as mean ± SEM, ** p < 0.01. (G,H) Classification of germ cells in respect to chromatin structure marked with <t>DAPI</t> staining in WT and Ankrd31 –/– testicular sections (7 μm). The elongated spermatids not correctly oriented toward seminiferous lumen are indicated by white arrowheads. (B–H) Scale bar corresponds to 50 μm; (D,E) scale bar corresponds to 25 μm.
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    Morphological characterization of Ankrd31 –/– testes. (A) Comparative size analysis between WT and Ankrd31 –/– testes. Testis weight (mg) analysis was carried out on WT and Ankrd31 –/– testes ( n = 20). Data are reported as mean value ± S.E.M. (B,C) Defective spermatogenesis was observed by H&E staining of WT and Ankrd31 –/– Bouin’s fixed seminiferous tubule sections (7 μm) collected from 8-week-old testes. SPG, spermatogonia; SPC, spermatocytes; rSPT, round spermatids; eSPT, elongated spermatids (black-arrowheads). (D,E) Cells in metaphase are indicated by white arrowheads. (F) Quantification of seminiferous tubules containing spermatocytes in metaphase in WT and Ankrd31 –/– testes ( n = 10 for each genotype). The number of total tubules counted in five different animals for each genotype corresponded to n = 650. Data are expressed as percentage (%) of tubules containing cells in metaphase and reported as mean ± SEM, ** p < 0.01. (G,H) Classification of germ cells in respect to chromatin structure marked with <t>DAPI</t> staining in WT and Ankrd31 –/– testicular sections (7 μm). The elongated spermatids not correctly oriented toward seminiferous lumen are indicated by white arrowheads. (B–H) Scale bar corresponds to 50 μm; (D,E) scale bar corresponds to 25 μm.
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    Morphological characterization of Ankrd31 –/– testes. (A) Comparative size analysis between WT and Ankrd31 –/– testes. Testis weight (mg) analysis was carried out on WT and Ankrd31 –/– testes ( n = 20). Data are reported as mean value ± S.E.M. (B,C) Defective spermatogenesis was observed by H&E staining of WT and Ankrd31 –/– Bouin’s fixed seminiferous tubule sections (7 μm) collected from 8-week-old testes. SPG, spermatogonia; SPC, spermatocytes; rSPT, round spermatids; eSPT, elongated spermatids (black-arrowheads). (D,E) Cells in metaphase are indicated by white arrowheads. (F) Quantification of seminiferous tubules containing spermatocytes in metaphase in WT and Ankrd31 –/– testes ( n = 10 for each genotype). The number of total tubules counted in five different animals for each genotype corresponded to n = 650. Data are expressed as percentage (%) of tubules containing cells in metaphase and reported as mean ± SEM, ** p < 0.01. (G,H) Classification of germ cells in respect to chromatin structure marked with DAPI staining in WT and Ankrd31 –/– testicular sections (7 μm). The elongated spermatids not correctly oriented toward seminiferous lumen are indicated by white arrowheads. (B–H) Scale bar corresponds to 50 μm; (D,E) scale bar corresponds to 25 μm.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Ankrd31 in Sperm and Epididymal Integrity

    doi: 10.3389/fcell.2021.741975

    Figure Lengend Snippet: Morphological characterization of Ankrd31 –/– testes. (A) Comparative size analysis between WT and Ankrd31 –/– testes. Testis weight (mg) analysis was carried out on WT and Ankrd31 –/– testes ( n = 20). Data are reported as mean value ± S.E.M. (B,C) Defective spermatogenesis was observed by H&E staining of WT and Ankrd31 –/– Bouin’s fixed seminiferous tubule sections (7 μm) collected from 8-week-old testes. SPG, spermatogonia; SPC, spermatocytes; rSPT, round spermatids; eSPT, elongated spermatids (black-arrowheads). (D,E) Cells in metaphase are indicated by white arrowheads. (F) Quantification of seminiferous tubules containing spermatocytes in metaphase in WT and Ankrd31 –/– testes ( n = 10 for each genotype). The number of total tubules counted in five different animals for each genotype corresponded to n = 650. Data are expressed as percentage (%) of tubules containing cells in metaphase and reported as mean ± SEM, ** p < 0.01. (G,H) Classification of germ cells in respect to chromatin structure marked with DAPI staining in WT and Ankrd31 –/– testicular sections (7 μm). The elongated spermatids not correctly oriented toward seminiferous lumen are indicated by white arrowheads. (B–H) Scale bar corresponds to 50 μm; (D,E) scale bar corresponds to 25 μm.

    Article Snippet: Nuclei were counterstained with DAPI II counterstain medium (Abbott Molecular Inc.).

    Techniques: Staining